Transcriptome sequence of Argopecten irradians SB68 - SRA

SRX22908511: Transcriptome sequence of Argopecten irradians SB68
1 ILLUMINA (Illumina NovaSeq 6000) run: 19.4M spots, 5.8G bases, 1.7Gb downloads

Design: RNA was extracted using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel, Dren, Germany). RNA quantity and quality were checked spectrophotometrically (NanoDrop ND-1000, Thermo Fisher Scientific, Wilmington, Delaware, USA). Library preparation and sequencing were performed by Novogene Corporation (UC Davis, Sacramento, California, USA). Sample quality control measures implemented by Novogene rely mainly on RNA Nano 6000 Assay Kit using the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, California, USA). RNASeq libraries were prepared using 1 g RNA using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts USA). Library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, California, USA). Then 3 l USER Enzyme (New England Biolabs, USA) was used with size-selected, adaptor-ligated cDNA at 37C for 15 min followed by 5 min at 95C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. Finally, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina)

Submitted by: Stony Brook University

Study: Argopecten irradians isolate:NY Genome sequencing

PRJNA1050236 • SRP478220 • All experiments • All runs

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This project presents the first chromosomal-level genome assembly of the bay scallop, Argopecten irradians, obtained from New York. The assembly provides a complete genomic profile of the species, offering critical insights into the evolutionary patterns and adaptive abilities of marine bivalves. Achieving this level of detail in the genome assembly enhances our understanding of molluscan genomic science and establishes a crucial tool for addressing environmental and pathogenic challenges in marine ecosystems.

Sample:

SAMN38861381 • SRS19877304 • All experiments • All runs

Organism: Argopecten irradians

Library:

Name: SB68

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 19.4M spots, 5.8G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR27230008	19,397,495	5.8G	1.7Gb	2024-06-05

ID:: 30970702

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